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Two subsets of dendritic cells, differing in T-cell stimulatory function, have been purified directly from human blood. Both subsets are positive for major histocompatibility complex MHC class II expression and negative for lineage-specific antigens e. The CD11c-negative subset is functionally immature, requiring monocyte-derived cytokines to develop into typical dendritic cells. The CD11c-positive subset has potent T-cell stimulating activity and expresses the activation antigen CD45RO, unlike its immature counterpart. However, these mature cells only develop typical dendritic morphology and high levels of MHC proteins and adhesins after a period of culture independent of exogenous cytokines. Although the freshly isolated mature dendritic cells resemble monocytes in cytospin preparations, the former lack CD14 and have a much stronger primary T-cell stimulatory capacity. We hypothesize that the CD11c-negative immature cells are marrow-derived precursors to tissue dendritic cells, such as epidermal Langerhans' cells, while the CD11c-positive cells are derived from tissues where they have been activated by antigen, and are en route to the spleen or lymph nodes to stimulate T-cell responses there.
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Differential responses between mature and young leaves of sunflower plants to oxidative stress caused by water deficit. Rural [online]. ISSN The effects of water stress and rehydration on leaf gas exchange characteristics along with changes in lipid peroxidation and pirogalol peroxidase PG-POD were studied in mature and in young leaves of sunflower Helianthus annuus L. Water stress reduced photosynthesis P n , stomatal conductance g s , and transpiration E in both young and mature leaves. However, the amplitude of the reduction was dependent on leaf age. The intercellular CO 2 concentration C i was increased in mature leaves but it was not altered in young leaves. Instantaneous water use efficiency WUE in mature stressed leaves was reduced when compared to control leaves while in young stressed leaves it was maintained to the same level as the control. After 24h of rehydration, most of the parameters related to gas exchange recovered to the same level as the unstressed plants except gs and E in mature leaves. However, after rehydration the enzyme activity was increased in mature leaves and remained to the same as the control in young leaves.
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Differential responses between mature and young leaves of sunflower plants to oxidative stress caused by water deficit. Rural [online]. ISSN The effects of water stress and rehydration on leaf gas exchange characteristics along with changes in lipid peroxidation and pirogalol peroxidase PG-POD were studied in mature and in young leaves of sunflower Helianthus annuus L. Water stress reduced photosynthesis P n , stomatal conductance g s , and transpiration E in both young and mature leaves. However, the amplitude of the reduction was dependent on leaf age.

The intercellular CO 2 concentration C i was increased in mature leaves but it was not altered in young leaves. Instantaneous water use efficiency WUE in mature stressed leaves was reduced when compared to control leaves while in young stressed leaves it was maintained to the same level as the control. After 24h of rehydration, most of the parameters related to gas exchange recovered to the same level as the unstressed plants except gs and E in mature leaves.

However, after rehydration the enzyme activity was increased in mature leaves and remained to the same as the control in young leaves. Malondialdehyde MDA content was increased by water stress in both mature and young leaves.

The results suggest that young leaves are more susceptible to water stress in terms of gas exchange characteristics than mature leaves although both went through oxidative estresse. Keywords : photosynthesis; antioxidant enzymes; lipid peroxidation; rehydration; Helianthus annuus. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

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